《玩具熊的午夜后宫：姐妹地点》（Five Nights at Freddy's: Sister Location，简称FNAF5）是Scott Cawthon在2016年发布的系列第五部作品。本作不再是单纯守办公室的模式，玩家每晚需要前往地下马戏团设施的不同房间完成维修任务，剧情深度挖掘威廉·阿夫顿的家庭悲剧，首次登场的埃纳德与马戏团宝贝成为系列人气角色，揭开了阿夫顿制造杀戮玩偶的真正目的。
Five Nights at Freddy's: Sister Location (FNAF 5) is the fifth installment created by Scott Cawthon and released in 2016. It abandons the classic static office defense. Every night, the player must travel between different rooms of the underground circus facility to perform maintenance tasks. The story digs deep into William Afton's family tragedy. The debut of Ennard and Circus Baby are fan favorites, revealing Afton's true motive for building the killer animatronics.
FNAF5故事发生在FNAF4之后，FNAF1之前。玩家名为迈克尔·阿夫顿，受人工语音HandUnit指引，在地下的马戏团宝贝娱乐设施执行夜班维护工作。这座地下设施由威廉·阿夫顿秘密建造，所有马戏团系列电子玩偶都内置了抓捕儿童的装置。迈克尔来到此地的目的，是寻找被囚禁在马戏团宝贝体内的妹妹伊丽莎白·阿夫顿。随着夜晚推进，设施内的玩偶们开始密谋，计划将它们的零件组合，借由迈克尔的身体逃离地下。
FNAF 5 takes place after FNAF4 and before FNAF1. The player is Michael Afton, guided by the AI HandUnit, working night shifts for maintenance inside the underground Circus Baby's Entertainment and Rental facility. This underground complex was secretly built by William Afton. All circus animatronics are equipped with devices to capture children. Michael comes here to find his sister Elizabeth Afton, who was trapped inside Circus Baby. As nights pass, the animatronics hatch a plan to merge their parts and escape the underground by using Michael's body.
马戏团宝贝（Circus Baby）：本作核心角色，外表是可爱的小女孩马戏团玩偶，体内囚禁着伊丽莎白·阿夫顿的灵魂。她拥有独立思考能力，策划了所有玩偶的逃跑计划，是埃纳德的主体。
Circus Baby: The core character of this game. A cute little girl circus animatronic containing Elizabeth Afton's soul. She can think independently and organized the escape plan, acting as the main part of Ennard.
芭蕾拉（Ballora）：芭蕾舞者玩偶，依靠听觉感知敌人，无法看见玩家。保持静止就不会被她发现，一旦移动发出声响就会被她追击。
Ballora: Ballerina animatronic that relies entirely on hearing. She cannot see the player. Stay still to avoid detection; moving and making noise triggers her chase.
欢乐时光霍斯（Funtime Foxy）：狐狸型欢乐时光玩偶，藏在表演幕布之后。玩家需要时刻监控表演时间，在他登场之前完成撤离。
Funtime Foxy: Funtime fox animatronic hiding behind curtains. Monitor showtime carefully and escape before he comes out to hunt.
欢乐时光邦尼（Funtime Freddy）：白色邦尼玩偶，手上附有邦邦玩偶Bon-Bon。会不断呼叫玩家名字，玩家需要控制邦邦关闭他体内的电源。
Funtime Freddy: White bunny animatronic with hand puppet Bon-Bon. He repeatedly calls the player's name. The player must use Bon-Bon to shut down his power.
埃纳德（Ennard）：由所有欢乐时光玩偶拆解、拼接组合而成的混合怪物，集合了所有玩偶的线路与眼球。在真结局中，埃纳德剥下迈克尔的外皮，伪装成人类逃出地下设施。
Ennard: A merged monster assembled from all Funtime animatronic parts, wires and eyes. In the true ending, Ennard skins Michael and escapes the facility disguised as a human.
本作补全了阿夫顿家族悲剧：威廉的女儿伊丽莎白不听警告，靠近马戏团宝贝，被玩偶内置的装置捕获身亡，灵魂寄宿在宝贝体内。多年之后，迈克尔来到地下设施寻找妹妹。所有欢乐时光玩偶联手融合成埃纳德，夺取迈克尔的皮囊逃到地面。普通结局中迈克尔被埃纳德抛弃，而真实结局揭示迈克尔没有死去，被身体内的病毒改造，成为不死之人，开始寻找父亲威廉·阿夫顿，这条故事线直接衔接FNAF6。
This game completes the Afton family tragedy. William’s daughter Elizabeth ignored warnings and approached Circus Baby. She was captured and killed by the animatronic, her soul trapped inside. Years later, Michael comes underground to find his sister. All Funtime animatronics combine into Ennard, take Michael’s skin and escape to the surface. In the normal ending Ennard abandons Michael. The true ending reveals Michael survives and is mutated, becoming immortal. He sets out to hunt his father William Afton, directly leading into FNAF6.